Optimization and Effects of Different Reaction Conditions for the Bioinspired Synthesis of Silver Nanoparticles Using Hippophae rhamnoides Linn. Leaves Aqueous Extract

The use of plant extracts for the synthesis of nanoparticles plays a significant role in nanotechnology field as it is simple, fast, environment friendly and no use of toxic chemicals. The current research work investigate the findings of optimization of different experimental variables conditions like reaction pH, silver nitrate concentrations, time, temperature and mixing ratio of the reactants on silver nanoparticles synthesized using aqueous extract of Hippophae rhamnoides L. (Seabuckthorn) leaves. The formation of yellowish brown color was confirmed the synthesis of silver nanoparticles. The optimized condition for the bioinspired synthesis of silver nanoparticles revealed that silver nitrate concentration was 1mM, temperature was 75°C, pH was 7, incubation time was 1 h and aqueous extract and silver nitrate ratio was 5:95. It can be observed from the finding that good modification of the bioprocess parameters will improve potential of desired nano-product for particular applications.